VITAL STAINING OF THE CENTRIFUGED ARBACIA PUNCTULATA EGG

¹ From the Marine Biological Laboratory, Woods Hole, and the Biological Laboratory, Princeton University

A table is given of the action of various vital dyes on the different materials in the centrifuged egg of Arbacia punctulata. The jelly surrounding the egg is stained with Janus green, Janus dark blue B, (purple) and toluidin blue (pinkish lavender). The clear layer is slightly stained with Bismarck brown and chrysoidin (yellow), Nile blue (blue), toluidin blue (pinkish lavender), rhodamine (pink), and neutral red (pinkish yellow). The mitochondrial layer is differentially stained with methyl green and gentian violet (purple) and Janus green (blue). Yolk and pigment are stained with brilliant cresyl blue, methylene blue and Nile blue (blue), toluidin blue (purple), rhodamine and neutral red (red), Bismarck brown and chrysoidin (yellow-brown). With safranin, the pigment is stained blood red, the yolk is unstained.