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1 From the Biological Laboratories, Harvard University and the Department of Biology, St. Anselm's College
1. Removal of both eyestalks of Cambarus virilis and Cambarus immunis induces the formation of gastroliths at times of the year when they would not normally form. Removal of one eyestalk fails to induce gastrolith formation.
2. Transplantation of sinus glands into the abdominal region of eyestalkless crayfish prevents the formation of gastroliths.
3. Temperatures of 15° C. and lower prevent or greatly retard the formation of gastroliths in eyestalkless crayfish.
4. As early as twenty-four hours after eyestalk removal marked histological changes occur in the regions of the stomach which secrete the gastroliths. There is proliferation and increase in height of the epithelial cells and they have already begun the secretion of the gastroliths.
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