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1 Edward Martin Biological Laboratory, SSwarthmore College, Swarthmore, Pennsylvania
A volumetric microrespirometer was used to measure the oxygen consumption of fox sperm obtained by electrical stimulation. Determinations were made on unmodified semen and on the components of semen during the first 1
-2 hours following ejaculation. After an initial high stabilization period, lasting up to 15 minutes, the oxygen uptake of sperm suspensions averaged 19 cu. mm./108 sperm/hour at 37°-38° C. The oxygen consumption was proportional to the number of sperm present over an eightfold range. Neither spermfree semen, preputial secretions, prostatic fluids, nor urine consumed oxygen if not contaminated by tissue debris.
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