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Biol Bull 85: 151-156. (October 1943)
© 1943 Marine Biological Laboratory
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THE SPERMATOZOON AND FERTILIZATION MEMBRANE OF ARBACIA PUNCTULATA AS SHOWN BY THE ELECTRON MICROSCOPE

ETHEL BROWNE HARVEY 1 and THOMAS F. ANDERSON 1

1 The Marine Biological Laboratory, Woods Hole; the Biological Laboratory, Princeton University; and the Eldridge Reeves Johnson Foundation for Medical Physics, University of Pennsylvania

1. As studied with the electron microscope, the tail of the Arbacia punctulata spermatozoon is found to disrupt into about ten distinct fibrils when it is washed in distilled water and dried. Each fibril is about 50 mµ in thickness. Regularly spaced cross striations also appear in the tail structure, but these may be produced in the washing and drying process.

2. A method of obtaining the fertilization membranes of Arbacia punctulata eggs free from egg material is described. When these were washed in distilled water and dried for examination, the electron microscope revealed no regular structures nor definite patterns. The thickness of the fertilization membrane, when first formed and dried is estimated to be of the order of 25 mµ.




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S. H. Wu and F. F. McKenzie
Electron Microscopic Study of Spermatozoa
Science, February 12, 1954; 119(3085): 212 - 213.
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