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Biol Bull 89: 229-241. (December 1945)
© 1945 Marine Biological Laboratory
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STUDIES ON THE BIOCHEMISTRY OF TETRAHYMENA. VII. RIBOFLAVIN, PANTOTHEN, BIOTIN, NIACIN AND PYRIDOXINE IN THE GROWTH OF T. GELEII W

GEORGE W. KIDDER 1 and VIRGINIA C. DEWEY 1

1 Arnold Biological Laboratory, Brown University, Providence, R. I.

1. It has been possible to prepare media for the growth of Tetrahymena geleii W which are free of riboflavin, pantothen, biotin, niacin, and pyridoxine, as determined by the Lactobacillus casei test.

2. T. geleii W is not dependent on an exogenous source of any one of the above vitamins. Omission of any one, however, reduces the maximum yield and, with the single exception of pantothen, the growth rate.

3. Biosynthesis of appreciable amounts of riboflavin, pantothen, and biotin occurs. These vitamins are found bound in the cell protoplasm. No significant increases of pyridoxine by biosynthesis were found.

4. The five vitamins listed are not essential growth factors for T. geleii W but are stimulatory factors, and as such should be included in the medium for optimum growth.






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