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Biol Bull 91: 141-144. (October 1946)
© 1946 Marine Biological Laboratory
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ELECTRON MICROSCOPE OBSERVATIONS OF THE TRICHOCYSTS AND CILIA OF PARAMECIUM

M. A. JAKUS 1 and C. E. HALL 1

1 Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts

Electron micrographs of shadow-cast trichocysts of Paramecium show that the dried trichocyst shaft is flattened on the supporting film, while the pointed tip is apparently more resistant to collapse on dehydration. Accentuation, by the metal, of the cross striation previously observed in the shaft indicates that the periodicity is accompanied by corrugation of the dried surface. A cross striation in the tip is also visible in some micrographs of shadow-cast specimens. In the few cases where the periodicity could be measured, the average spacing was about 300 A, as compared to about 550 A for the well-defined shaft striation.

In electron micrographs of shadow-cast specimens of Paramecium cilia, the component fibrils are seen with greatly increased clarity.




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