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1 The Marine Biological Laboratory, Woods Hole, Massachusetts and the Department of Biology, Queens College, Flushing, New York
1. Spawning occurred in the sea-cucumber, Thyone briareus, during the month of June, 1948. The phenomenon appeared to wane as the month progressed. Probably early June and possibly even earlier months are the best time to find high percentages of ripe germ cells.
2. The process of shedding is described, together with various details concerning the handling of shedding animals and their gametes.
3. The following possible factors in shedding are discussed: Time of day, amount of light, presence of a shedding male to induce shedding by the female, size, quantity sea water, freshness of animals, temperature, etc. It is shown that the first three are not essential prerequisites for shedding.
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