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Biol Bull 96: 70-73. (February 1949)
© 1949 Marine Biological Laboratory
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STRATIFICATION AND DEFORMATION OF ARBACIA PUNCTULATA EGGS CENTRIFUGED IN CAFFEINE SOLUTIONS

RALPH HOLT CHENEY 1

1 Brooklyn College of the City of New York and the Marine Biological Laboratory, Woods Hole, Massachusetts

1. Caffeine does not change the existing viscosity state of the egg.

2. Egg fragmentation, under centrifugation, decreases with increased caffeine concentration.

3. The "apparent" effect of greater stratification of the granules in Arbacia eggs centrifuged in caffeine does not occur if the centrifugal force to which the eggs are subjected is sufficient to produce sedimentation but insufficient to cause deformation.

4. Evidence indicates that the delay of deformation in the caffeinized eggs, centrifuged at 10,000 x g or less, may be due to the action of caffeine (trimethylated purine) upon the total tension forces at the surface areas of both unfertilized and fertilized Arbacia eggs.






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Copyright © 1949 by the Marine Biological Laboratory.