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Biol Bull 96: 233-237. (June 1949)
© 1949 Marine Biological Laboratory
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PIPERAZINE DIHYDROCHLORIDE AND GLYCYLGLYCINE AS NON-TOXIC BUFFERS IN DISTILLED WATER AND IN SEA WATER

MARSHALL E. SMITH 1 and LYNWOOD B. SMITH 1

1 The Marine Biological Laboratory, Woods Hole, Mass.

The advantages of piperazine dihydrochloride and glycyllycine as buffers include the low toxicity, the lack of volatility, the solubility, the availability of the pure products, the convenience and accuracy of buffer preparation, and the lack of precipitation of calcium and magnesium salts from sea water below a pH of 9.9.

Table I indicates the preparation of solutions of known pH involving piperazine dihydrochloride, glycylglycine, and mixtures of these two in distilled water and in sea water at 25° C. Because the determinations were not made with the hydrogen electrode this table must be considered as being susceptible to small error, especially on the alkaline side of pH 9.

Table II shows the pK1' and pK2' values for piperazine dihydrochloride for several ionic strengths. Using the Debye-Hückel equation, the extrapolated pK values of piperazine at infinite dilution were found to be 5.32 for pK1 and 9.70 for pK2.






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Copyright © 1949 by the Marine Biological Laboratory.