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1 Department of Zoology, University of Missouri, Columbia, Mo.
1. At 5° C. about 50 per cent mortality of Drosophila melanogaster is reached after two hours; at 10° C. a 20 minute exposure kills very few whereas a 25 minute exposure is almost completely lethal; at 15° C. about 50 per cent survive exposures less than 10 minutes long whereas an exposure of 13 minutes or longer is completely lethal; and at 20° C. all individuals are killed within a few minutes.
2. Cold shocks of air at 5° C. for 75 to 90 minutes and at 10° C. for 5 to 20 minutes are lethal to sperm stored in adult females although such treatment has no effect on the subsequent fertility of such females. Males are not sterilized to any great extent by such exposures.
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