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Figure 1. Immunostaining intensity levels for calexcitin under two experimental conditions. First, specimens of Hermissenda were exposed to 10 µg/ml of RGD, a tripeptide, cell adhesion molecule inhibitor, at the designated post-training (nine training events) application times through to 240 min (designated RGD.x (min); double-hatched columns). At 240 min, the animals were tested, the RGD removed, and the animals transferred in the training tray to a tub with running seawater being siphoned through each lane until they were tested again at 24-h post-training. Second, replicate sets of animals (solid black columns) were similarly trained but not exposed to RGD or tested, and then decapitated and fixed at time points similar to those for the RGD-exposed animals. An overall control group (single-hatched column) consisted of naive, untrained animals held under similar conditions until they were also decapitated and fixed. All central nervous systems were processed collectively for immunocytochemistry. Calexcitin immunostaining intensity was measured from digital photomicrographs using NIH Image software. Results were statistically compared using ANOVA and pairwise Student’s t tests (see text).
